| Brad Epperly | BNSF Railway Co. | Opposing | GA89 |
| Dustin Miller | BNSF Railway Co. | Opposing | GA89 |
| Jim Obradovich | Boone and Scenic Valley Railroad | Opposing | GA89 |
| Robert Mulqueen | Boone and Scenic Valley Railroad | Opposing | GA89 |
| Jim Obradovich | Burlington Junction Railway | Opposing | GA89 |
| Robert Mulqueen | Burlington Junction Railway | Opposing | GA89 |
| Kate Walton | CN Rail | Opposing | GA89 |
| Madeleine Bradley | CN Rail | Opposing | GA89 |
| Matt Eide | CN Rail | Opposing | GA89 |
| Kellie Paschke | Iowa Cattlemen's Association | Supporting | GA89 |
| Kelly Meyers | Iowa Cattlemen's Association | Supporting | GA89 |
| Logan Murray | Iowa Cattlemen's Association | Supporting | GA89 |
| Troy Skinner | Iowa Cattlemen's Association | Supporting | GA89 |
| Lyle Brehm | Iowa County Engineers Association | Opposing | GA89 |
| Emilee Harris-Puricelli | Iowa Farmers Union | Undecided | GA89 |
| Nick Laning | Iowa Farmers Union | Undecided | GA89 |
| Threase Harms | Iowa Farmers Union | Undecided | GA89 |
| Jim Obradovich | Iowa Interstate Railroad | Opposing | GA89 |
| Robert Mulqueen | Iowa Interstate Railroad | Opposing | GA89 |
| Mike Heller | Iowa Renewable Fuels Association (IRF) | Undecided | GA89 |
| Jamie Cashman | Iowa State Association of Counties | Undecided | GA89 |
| Kristi Harshbarger | Iowa State Association of Counties | Undecided | GA89 |
| Lucas Beenken | Iowa State Association of Counties | Undecided | GA89 |
| William Peterson | Iowa State Association of Counties | Undecided | GA89 |
| Jamie Cashman | Iowa State Association of County Supervisors | Undecided | GA89 |
| Kristi Harshbarger | Iowa State Association of County Supervisors | Undecided | GA89 |
| Lucas Beenken | Iowa State Association of County Supervisors | Undecided | GA89 |
| William Peterson | Iowa State Association of County Supervisors | Undecided | GA89 |
| Gary Grant | Linn County Board of Supervisors | Opposing | GA89 |
| Gary Grant | Linn County Board of Supervisors | Withdrawn | GA89 |
| Gary Grant | Linn County Board of Supervisors | Undecided | GA89 |
| Larry Murphy | Linn County Board of Supervisors | Opposing | GA89 |
| Larry Murphy | Linn County Board of Supervisors | Withdrawn | GA89 |
| Larry Murphy | Linn County Board of Supervisors | Undecided | GA89 |
| Meghan Malloy | Linn County Board of Supervisors | Opposing | GA89 |
| Meghan Malloy | Linn County Board of Supervisors | Withdrawn | GA89 |
| Meghan Malloy | Linn County Board of Supervisors | Undecided | GA89 |
| Gary Grant | Urban County Coalition | Opposing | GA89 |
| Gary Grant | Urban County Coalition | Withdrawn | GA89 |
| Gary Grant | Urban County Coalition | Undecided | GA89 |
| Larry Murphy | Urban County Coalition | Opposing | GA89 |
| Larry Murphy | Urban County Coalition | Withdrawn | GA89 |
| Larry Murphy | Urban County Coalition | Undecided | GA89 |
| Meghan Malloy | Urban County Coalition | Opposing | GA89 |
| Meghan Malloy | Urban County Coalition | Withdrawn | GA89 |
| Meghan Malloy | Urban County Coalition | Undecided | GA89 |