| Brad Epperly | BNSF Railway Co. | Opposing | GA91 |
| Brad Epperly | BNSF Railway Co. | Undecided | GA91 |
| Casey Nickel | BNSF Railway Co. | Opposing | GA91 |
| Casey Nickel | BNSF Railway Co. | Undecided | GA91 |
| Dustin Miller | BNSF Railway Co. | Opposing | GA91 |
| Dustin Miller | BNSF Railway Co. | Undecided | GA91 |
| Jefferson Fink | Boone and Scenic Valley Railroad | Opposing | GA91 |
| Jim Obradovich | Boone and Scenic Valley Railroad | Opposing | GA91 |
| Victoria Sinclair | Boone and Scenic Valley Railroad | Opposing | GA91 |
| Ross Grooters | Brotherhood of Locomotive Engineers and Trainmen | Supporting | GA91 |
| Jefferson Fink | Burlington Junction Railway | Opposing | GA91 |
| Jim Obradovich | Burlington Junction Railway | Opposing | GA91 |
| Victoria Sinclair | Burlington Junction Railway | Opposing | GA91 |
| Kate Walton | CN Rail | Undecided | GA91 |
| Madeleine Bradley | CN Rail | Undecided | GA91 |
| Matt Eide | CN Rail | Undecided | GA91 |
| Jefferson Fink | Davenport Industrial Railroad | Opposing | GA91 |
| Jim Obradovich | Davenport Industrial Railroad | Opposing | GA91 |
| Victoria Sinclair | Davenport Industrial Railroad | Opposing | GA91 |
| Brad Hartkopf | Iowa Association of Business and Industry (ABI) | Opposing | GA91 |
| John Davis | Iowa Association of Business and Industry (ABI) | Opposing | GA91 |
| Nicole Crain | Iowa Association of Business and Industry (ABI) | Opposing | GA91 |
| Sara Throener | Iowa Department of Inspections, Appeals, and Licensing | Undecided | GA91 |
| Sarah Vanderploeg | Iowa Department of Inspections, Appeals, and Licensing | Undecided | GA91 |
| Jefferson Fink | Iowa Interstate Railroad | Opposing | GA91 |
| Jim Obradovich | Iowa Interstate Railroad | Opposing | GA91 |
| Victoria Sinclair | Iowa Interstate Railroad | Opposing | GA91 |
| Cody Carlson | National Federation of Independent Business (NFIB /IA) | Opposing | GA91 |
| Chris Smith | SMART-Transportation Division | Supporting | GA91 |
| Larry Lloyd | Soo Line Railroad Company d/b/a Canadian Pacific Kansas City (CPKC) | Undecided | GA91 |
| Michael Triplett | Union Pacific Railroad | Opposing | GA91 |