| Brad Epperly | BNSF Railway Co. | Undecided | GA91 |
| Casey Nickel | BNSF Railway Co. | Undecided | GA91 |
| Dustin Miller | BNSF Railway Co. | Undecided | GA91 |
| Jefferson Fink | Boone and Scenic Valley Railroad | Undecided | GA91 |
| Jim Obradovich | Boone and Scenic Valley Railroad | Undecided | GA91 |
| Victoria Sinclair | Boone and Scenic Valley Railroad | Undecided | GA91 |
| Ross Grooters | Brotherhood of Locomotive Engineers and Trainmen | Supporting | GA91 |
| Jefferson Fink | Burlington Junction Railway | Undecided | GA91 |
| Jim Obradovich | Burlington Junction Railway | Undecided | GA91 |
| Victoria Sinclair | Burlington Junction Railway | Undecided | GA91 |
| Kate Walton | CN Rail | Undecided | GA91 |
| Madeleine Bradley | CN Rail | Undecided | GA91 |
| Matt Eide | CN Rail | Undecided | GA91 |
| Barbra Solberg | Cedar Rapids Metro Economic Alliance | Undecided | GA91 |
| Gary Grant | Cedar Rapids Metro Economic Alliance | Undecided | GA91 |
| Jefferson Fink | Davenport Industrial Railroad | Undecided | GA91 |
| Jim Obradovich | Davenport Industrial Railroad | Undecided | GA91 |
| Victoria Sinclair | Davenport Industrial Railroad | Undecided | GA91 |
| Clare Gill | Dubuque Area Chamber of Commerce | Supporting | GA91 |
| Matt Ohloff | Environmental Law and Policy Center | Supporting | GA91 |
| Jacob Schrader | Greater Des Moines Partnership | Undecided | GA91 |
| Logan Murray | Greater Des Moines Partnership | Undecided | GA91 |
| Ryan Moon | Greater Des Moines Partnership | Undecided | GA91 |
| Mackenzie DeRoo | Greater Iowa City, Inc. | Supporting | GA91 |
| Jim Garrett | Iowa Association of Railroad Passengers | Supporting | GA91 |
| Pam Mackey-Taylor | Iowa Chapter of Sierra Club | Supporting | GA91 |
| Jefferson Fink | Iowa Interstate Railroad | Undecided | GA91 |
| Jim Obradovich | Iowa Interstate Railroad | Undecided | GA91 |
| Victoria Sinclair | Iowa Interstate Railroad | Undecided | GA91 |
| Amy Campbell | Professional Developers of Iowa (PDI) | Undecided | GA91 |
| Chloe Gayer | Professional Developers of Iowa (PDI) | Undecided | GA91 |
| Craig Patterson | Professional Developers of Iowa (PDI) | Undecided | GA91 |
| Ryan Sempf | Quad Cities Chamber of Commerce | Supporting | GA91 |
| Chris Smith | SMART-Transportation Division | Supporting | GA91 |
| Michael Triplett | Union Pacific Railroad | Undecided | GA91 |